[Taxacom] DNA extraction from herbarium specimens

[Vitor Fernandes de Miranda]

Dear friends,

 

I've experimented to extract DNA from herbarium specimens, but the results are always disappointing. 

DNAzol and CTAB methods were tried but with no success.

 

Please, does anyone have any suggestion? Any protocol or even clues will be very welcome.

 

Best wishes,

V?tor

 

 

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Prof.Dr. V?tor Fernandes Oliveira de Miranda
Curador HUMC
Universidade de Mogi da Cruzes - UMC
Laborat?rio de Sistem?tica Vegetal
Herbarium Mogiense - HUMC (Sala 21.16 - Pr?dio II)
Av. Dr. C?ndido Xavier de Almeida Souza, n.200
CEP 08780-911 - Moji das Cruzes - SP - Brasil
Tel.: +55 (11) 4798-7000 Ramal 7313
http://www.umc.br/~vmiranda

e-mail: vmiranda at umc.br